Journal: Frontiers in Cell and Developmental Biology

Article Title: Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy

doi: 10.3389/fcell.2021.737621

Figure Lengend Snippet: Fluorescence images of immuno-labeled mouse cerebellum imaged with conventional FM and ILEM system (JEOL BSE detector). (A) An 80 µm slice of cerebellum labeled with calbindin D28 K primary and Alexa 488-conjugated secondary antibody before embedding with our IRF protocol. Imaged with conventional FM Nikon TiE inverted C2 confocal microscope Plan Apo 10x dry lens, labeled Purkinje cells can be easily recognized (asterisks). (B) After embedding with our IRF protocol, 150-nm cerebellar section imaged by conventional FM Nikon TiE inverted C2 confocal microscope Plan Apo 20x dry lens shows that the fluorescent labeling of Purkinje cells (asterisks) was preserved after embedding. (C) The same 150 nm section with a layer of Purkinje cells ( asterisk, in B) imaged with fluorescent optics of ILEM-SEM (Plan Apo VC 100x lens) system shows a perfect correlation. Abbreviations: FM, fluorescence microscope; ILEM-SEM, integrated light and electron microscope; IRF, in-resin fluorescence; PC, Purkinje cell. Scale bars: A = 100 μm, B = 10 μm, C = 5 µm.

Article Snippet: ITO coverslips with sections were mounted to a holder that could be fitted onto the SECOM stage (DELMIC, Delft, Netherlands), which in turn was mounted to a door that would fit the SEM (JSM 7200F LV, JEOL, Japan), comprising our ILEM.

Techniques: Fluorescence, Labeling, Microscopy

Journal: Frontiers in Cell and Developmental Biology

Article Title: Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy

doi: 10.3389/fcell.2021.737621

Figure Lengend Snippet: Three consecutive sections of mouse cerebellum immunolabeled for calbindin D28 K and imaged with ILEM-SEM system (JEOL BSE detector). (A–B, left) Same two large fluorescent Purkinje cells as in the center of , in two immediately adjacent consecutive 150 nm sections without any additional post-staining, imaged at higher magnifications with ILEM-SEM in FM mode with Plan Apo VC 100x lens. (A–B, middle and right) ILEM EM mode images indicated by white and black boxes in A–B, left. Purkinje cell boundaries (A–B , middle) , granular cells (B , middle) and three cross-sectioned dendrites (A , right) are demarcated by a dotted line for ease of recognition. (C , middle and right) Higher magnifications ILEM-SEM images from the regions indicated by white and black boxes in (C, left) . The images in C (middle and right) represent next consecutive section at the same place as in 8 B (middle and right) , but after post-staining with uranyl acetate and lead citrate. Post-staining enhanced contrast, so that the mitochondria, membranes, the secretory pathway organelles in PC cytoplasm, and climbing fiber synapse ( asterisk in B middle as compared C middle) are now well distinguished. Abbreviations: Cyt, cytoplasm; dd, dendrite; ILEM-SEM, integrated light and electron microscope; IRF, in-resin fluorescence; GC, granular cell; Nuc, nucleus; PC, Purkinje cell. Scale bars: (A–C), left = 10 μm; (A–C) , middle = 0.6 µm; (A–C) , right = 0.8 µm.

Article Snippet: ITO coverslips with sections were mounted to a holder that could be fitted onto the SECOM stage (DELMIC, Delft, Netherlands), which in turn was mounted to a door that would fit the SEM (JSM 7200F LV, JEOL, Japan), comprising our ILEM.

Techniques: Immunolabeling, Staining, Microscopy, Fluorescence

Journal: Journal of Computers in Education

Article Title: Metaverse system adoption in education: a systematic literature review

doi: 10.1007/s40692-022-00256-6

Figure Lengend Snippet:

Article Snippet: Sixth, it was discovered that SmartPLS (PLS-SEM) is an effective tool for validating metaverse models.

Techniques: Diffusion-based Assay, Software